Course Overview

Commercial Production of Orchids Through Tissue Culture 

Course Code: MC55007
Course Title: Commercial Production of Orchids Through Tissue Culture
Start Date: September 20, 2025 📅
Duration: 26 hours (13 weeks, 2 hours/week) ⏰
Mode: Primarily in-person, with occasional online sessions 🌐
Class Size: 10-30 participants 👥
Course Equivalent: 2 Credit Hours
Course Fee: 5,000 BDT (Special Offer: 3,000 BDT with 40% discount) 💸

Course Description 🚀

Commercial Production of Orchids Through Tissue Culture (MC55007) addresses the surging demand for high-quality orchids in Bangladesh, with a 30% market increase reported by the Bangladesh Florist Association (2022). This course equips learners with hands-on skills in orchid micropropagation using tissue culture, focusing on standardized protocols to meet commercial standards. Through practical training and entrepreneurial guidance, participants will be prepared for careers in floriculture, biotechnology, or agri-entrepreneurship, contributing to Bangladesh’s growing $50M floriculture industry (BARI, 2021). 🌺

Syllabus 📚

• Week 1: Introduction to Orchids & Tissue Culture – Orchid significance, tissue culture overview. Activities: Analyze market trends. Resources: Reference books. 🌸

• Week 2: Orchid Biology – Morphology, life cycle, explant sources. Activities: Identify orchid parts. Resources: Microscope, slides. 🌱

• Week 3: Lab Setup – Lab zoning, equipment overview. Activities: Tour lab setup. Resources: Laminar airflow cabinet, autoclave. 🧪

• Week 4: Media Preparation – Growth regulators, nutrients, pH adjustment. Activities: Prepare MS media. Resources: pH meter, chemicals. 🧫

• Week 5: Sterilization Techniques – Autoclaving, chemical sterilization. Activities: Sterilize explants. Resources: Bleach, ethanol. 🧴

• Week 6: Tissue Culture Process – Explant initiation, shoot multiplication. Activities: Initiate culture. Resources: Incubator. 🌿

• Week 7: Culture Maintenance – Contamination control, hardening. Activities: Monitor cultures. Resources: Humidity chambers. 🛡️

• Week 8: Hardening & Acclimatization – Plantlet transfer, nutrient management. Activities: Practice hardening. Resources: Substrates. 🌱

• Week 9: Field Transfer – Substrates, post-transfer care. Activities: Transfer plantlets. Resources: Greenhouse materials. 🌍

• Week 10: Entrepreneurship – Market analysis, costing, branding. Activities: Draft business plan. Resources: Case studies. 💼

• Week 11: Project Writing – Develop entrepreneurial project proposal. Activities: Write project plan. Resources: Templates. 📝

• Week 12: Field Visit – Observe commercial orchid production. Activities: Visit nursery. Resources: Transport. 🚐

• Week 13: Revision & Assessment – Review, final project submission. Activities: Present project. Resources: Evaluation rubrics. 🎯

Entry Requirements 📋

• Higher Secondary Certificate (HSC) or equivalent.

• Background in Biotechnology, Microbiology, Agriculture, or related fields.

• Basic understanding of plant biology, microbiology, and lab safety.

• Minimum age: 19 years.

Career Pathways 🚀

• Tissue culture specialist, orchid nursery manager, floriculture consultant.

• Agri-entrepreneur in orchid production or export businesses.

• Higher studies in Plant Biotechnology, Horticulture, or Agricultural Sciences.

• Professional Certificate in Orchid Tissue Culture.